DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Asthma: Lung, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Magbio Genomics Inc HighPrep Plasmid DNA Kit (96 preps)
Magbio's HighPrep™ Plasmid DNA kit is a magnetic beads based plasmid purification system for high throughput rapid isolation of high and low copy number plasmid DNA, BACs, PACs Cosmid and Fosmids. The system can be easily automated on many liquid handling workstation providing robustness and high quality plasmid DNA for subsequent downstream applications such as digestion and fluorescent DNA sequencing
Biochain Institute Inc SeqPure PCR Purification Kit (50 ml), 50 ml/pk
SeqPure PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.
Biochain Institute Inc Genomic DNA - Human Adult Normal Tissue: Heart: Ventricle, left, from a single donor, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Human Adult Normal Tissue: Uterus, from a single donor, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Bioworld BAC DNA MiniPrep Kit, 25 Prep(s)
For the rapid preparation of BAC DNA High DNA recovery Alcohol-free protocol Convenient spin column protocol FEATURES AND BENEFITSHigh Recovery -0.6 to 1 µg of BAC DNA can be purified from 3.0 mL of bacterial culture. Alcohol-free protocol -No alcohols are used durig the purification process, thus the DNA is compatible with alcohol sensitive downstream applications. Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 1 hour. Recovered DNA is suitable for downstream applications - Purified BAC DNA is fully compatible with restriction enzyme digestions and sequencing. High binding capacity of columns -The binding capacity of the columns in the BAC DNA MiniPrep Kit is 25 µg
Qiagen, Inc. GENERATION DNA ELUTION
Generation DNA Elution Solution (500 ml)
Qiagen, Inc. QIAAMP DNA BLOOD MAXI
NC3878372 QIAAMP DNA BLOOD MAXI
![Zymo Research Corporation ZymoBIOMICS 96 DNA Kit (2 x 96 preps)Includes: [D4307] ZymoBIOMICS 96 DNA Kit x 1, [S6002-96-4] ZR BashingBead Lysis Racks (0.1mm & 0.5 mm) 2 pack x 1[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-D4303-ZYMOBIOMICS-96-DNA-KIT.JPG-250.jpg)
Zymo Research Corporation ZymoBIOMICS 96 DNA Kit (2 x 96 preps)Includes: [D4307] ZymoBIOMICS 96 DNA Kit x 1, [S6002-96-4] ZR BashingBead Lysis Racks (0.1mm & 0.5 mm) 2 pack x 1[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1
The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies1 of different organisms (e.g. Gram-negative/positive bacteria including endospores2 , fungi, protozoans, algae, etc.) making it ideal for microbial community profiling. Unbiased mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads™ and validated using the ZymoBIOMICS™ Microbial Community Standard.In addition, the ZymoBIOMICS™ 96 DNA Kit is equipped with Zymo Researchs proprietary OneStep™ PCR InhibitorRemoval technology, enabling PCR from the most PCR prohibitive environmental samples rich in humic and fulvic acids, tannins, melanin, and other polyphenolic compounds. Coupling state-of-the-art lysis technology with Zymo-Spin™ Technology results in superior yields of ultra-pure DNA ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing.
ATCC Quantitative Genomic DNA from; Enterobacter cloacae; subsp. cloacae; Strain CDC 442-68
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Zymo Research Corporation ZR-96 BashingBead™ Lysis Rack (2 mm) (2 racks)
Tubes are in a 96-well rack with caps and a cover for high throughput processing. The state of the art, ultra-high density beads are fracture resistant, chemically inert, and ideal for disrupting tough-to-lyse biological samples. These beads are ideal for microbes and fungi in soil, feces, sludge, etc.
Zymo Research Corporation Quick DNA Universal Microprep Kit (50 Preps)
For high yield total DNA extraction (e.g., genomic, mitochondrial, viral), cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA > 50 kb to be eluted in as little as 10 l. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Zymo Research Corporation ZymoPURE Wash 2 (Concentrate), 28 ml
ZymoPURE™ Wash 2 Concentrate (28 ml). Assists in washing ZymoPURE™ columns for subsequent purification of nucleic acid.
Zymo Research Corporation ZymoPURE™ P2 (Green) (410 ml)
ZymoPURE P2 (Blue) is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
Zymo Research Corporation ZymoBIOMICS™ Microbial Community DNA Standard (200 ng)
One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other artifacts after the step of nucleic acid purification. This DNA standard is created by pooling DNA extracted from pure cultures. It has an accurately defined composition, negligible impurities (0.01%), and contains genomes of a wide range of GC content (15% - 85%). This DNA standard is designed to have the same microbial composition with the cellular version, the ZymoBIOMICS Microbial Community Standard, so that they can be more powerful when working in tandem.